FIG 2.

Northern blot validation of selected genes with altered expression in the presence of CHP in C. violaceum. Total RNA was extracted from wild-type strain ATCC 12472 grown in LB medium (control [CN]) or after exposure to 100 μM cumene hydroperoxide for 10 min (CHP). RNA was separated on a 1.5% agarose gel, transferred onto a nylon membrane, and hybridized with radiolabeled probes specific for each indicated gene (black arrows). Genes indicated in black or gray arrows were differentially expressed in the microarray analysis. White arrows indicate neighboring genes. We validated 11 upregulated genes (red vertical arrow) and six downregulated genes (green vertical arrow). Levels of rRNA were used as a loading control (bottom gel).