TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| C. violaceum strains | ||
| ATCC 12472 | Wild-type strain (sequenced genome) | 21 |
| JF0209 | Wild-type strain with the CV_0209 gene deleted (ΔohrA) | 17 |
| JF0210 | Wild-type strain with the CV_0210 gene deleted (ΔohrR) | 17 |
| JF0208I | Insertion mutant for the CV_0208 gene (CV0208::pNPT) | This study |
| JF1106I | Insertion mutant for the CV_1106 gene (trxC::pNPT) | This study |
| JF1107I | Insertion mutant for the CV_1107 gene (gpx::pNPT) | This study |
| JF2037I | Insertion mutant for the CV_2037 gene (garB::pNPT) | This study |
| JF3501I | Insertion mutant for the CV_3501 gene (nemA::pNPT) | This study |
| JF3549I | Insertion mutant for the CV_3549 gene (katE::pNPT) | This study |
| JF02100208I | Double mutant for CV_0210-CV_0208 (ΔohrR CV0208::pNPT) | This study |
| Plasmids | ||
| pNPTS138 | Suicide vector containing oriT sacB; Kanr | D. Alley |
| pMR20 | Low-copy-number and broad-host-range vector; oriT; Tcr | 67 |
| pMR-ohrR | pMR20 with the complete CV_0210 gene | 17 |