TABLE 3.
Primers used in this work
| Primer | Sequence (5′→3′)a | Description |
|---|---|---|
| Construction of mutant strains | ||
| CV1106MutFW | CCTAGCAAGCTTAGAAGGGCTGGTCATCCTGG | HindIII/BamHI fragment with 173 bp of the coding region |
| CV1106MutRv | GGCCTAGGATCCGCGTCGGAATGGAGCGGATG | |
| CV1106MutConf | CGACTATCGGCAGGATTG | |
| CV1107MutFW | CCTAGCAAGCTTCACGCCGCAGTTCACGCAAC | HindIII/BamHI fragment with 146 bp of the coding region |
| CV1107MutRv | GGCCTAGGATCCCGCCGTAATTGGCCTGGCAG | |
| CV1107MutConf | GCAATGTGAGACGATAGG | |
| CV2037MutFW | CCTAGCAAGCTTCGATGACCACACCATCGTCC | HindIII/BamHI fragment with 300 bp of the coding region |
| CV2037MutRv | GGCCTAGGATCCAACTGCTCGGACAAGGCCTG | |
| CV2037MutConf | GGCCACACTGACATGTTC | |
| CV3501MutFW | CCTAGCAAGCTTGCTGCAACTGTGGCACACCG | HindIII/BamHI fragment with 411 bp of the coding region |
| CV3501MutRv | GGCCTAGGATCCGAGATGCGGATGCCGACGTG | |
| CV3501MutConf | TGGCCAAGGAATACTACC | |
| CV3549MutFW | CCTAGCAAGCTTAGTTCTACACCGAGGAGGGC | HindIII/BamHI fragment with 480 bp of the coding region |
| CV3549MutRv | GGCCTAGGATCCTGAAGGGACAAGCGTCGGCC | |
| CV3549MutConf | GGCAAAAAGACCGACCTG | |
| CV0208MutFW | CCTAGCAAGCTTCCAACCGCAACCTGTTCTAC | HindIII/BamHI fragment with 157 bp of the coding region |
| CV0208MutRv | GGCCTAGGATCCATCAACTGCAGCACGCGGTC | |
| CV0208MutConf | CAGTTGATCGGTCTGCTG | |
| M13 forward | GTAAAACGACGGCCAGT | |
| Probes for EMSAs | ||
| pCV0208-Fw | TTGGATCCCAGATTCACGCGCTGCGCGC | BamHI/PstI fragment with 233 bp of the promoter region |
| pCV0208-Rv | ATTACTGCAGGAGCGGCTGGCGATGGACAG | |
| CV0208CDS-Fw | TTGGATCCGAAGGCCAGGCGCTGCATCT | BamHI/PstI fragment with 243 bp of the coding region (CDS) |
| CV0208CDS-Rv | ATTACTGCAGCTGTCGCTGCTGCGCACGAA | |
| pCV0209-Fw | TTGGATCCTGCCGGAGCGGAATGCAACT | BamHI/PstI fragment with 212 bp of the promoter region |
| pCV0209-Rv | ATTACTGCAGGCGGTGGCTTCGGCGGTATAC | |
| pCV0362-Fw | TTGGATCCGTCCTGCTTGACGTTTGAGAC | BamHI/PstI fragment with 371 bp of the promoter region |
| pCV0362-Rv | ATTACTGCAGCAAGGGCTTGCCGGCGGAGA | |
| pCV1444-Fw | TTGGATCCCTCTGGACTGATCCGCCCAG | BamHI/PstI fragment with 270 bp of the promoter region |
| pCV1444-Rv | ATTACTGCAGCATCATGAGCGGACTCCCGG | |
| pCV2001-Fw | TTGGATCCGCGAAGCGCGGCATTGAGGT | BamHI/PstI fragment with 435 bp of the promoter region |
| pCV2001-Rv | ATTACTGCAGGGCAGGCCGTGCGGGTAGTC | |
| Probes for Northern blot assays | ||
| CV_0208NB-Fw | GACCATACTAGACAGCTACGCC | Fragment with 115 bp of the coding region |
| CV_0208NB-Rv | CAGCGTGGTGATTTGCGGATAG | |
| CV_0209NB-Fw | TGCAGGTGAAGTTGAGCACCCC | Fragment with 103 bp of the coding region |
| CV_0209NB-Rv | CCGATGAAGCAGGCGGAATAGCC | |
| CV_1106NB-Fw | CAAGATGTTTGGCCCGGTGTTC | Fragment with 115 bp of the coding region |
| CV_1106NB-Rv | GGAGCGGATGTTGAAGTGGGC | |
| CV_1483NB-Fw | GCTGCTGAACGATATGTGGGG | Fragment with 118 bp of the coding region |
| CV_1483NB-Rv | GCTGTAGCGCCATTTGACCAG | |
| CV_2037NB-Fw | GCCTTGTCCGAGCAGTTCTAT | Fragment with 110 bp of the coding region |
| CV_2037NB-Rv | TCGACCTCTTCTCCGTCCAG | |
| CV_2241NB-Fw | TCCAAGACCCATTGAGCCG | Fragment with 123 bp of the coding region |
| CV_2241NB-Rv | CATCGGACGGAATCAGGCC | |
| CV_3549NB-Fw | GGCAACTGGGACCTCGTAG | Fragment with 114 bp of the coding region |
| CV_3549NB-Rv | GTCGCGCATATTGGTGTACG | |
| CV_3668NB-Fw | GACTTCGTGGTGCAGGTTCTC | Fragment with 117 bp of the coding region |
| CV_3668NB-Rv | GTCTTTGGTATAAACGCCACAC | |
| CV_3896NB-Fw | GACCTTCCCCAGCAAGACCTTC | Fragment with 124 bp of the coding region |
| CV_3896NB-Rv | CTTGTAATGGTCCCAGCGCAGC | |
| CV_4003NB-Fw | GACCTTCCAGATGACCTTCGC | Fragment with 121 bp of the coding region |
| CV_4003NB-Rv | CACATAGACCAGCAGAGACCAC | |
| CV_4014NB-Fw | CATCTCCGCCAACTCCGATAG | Fragment with 118 bp of the coding region |
| CV_4014NB-Rv | GTCCAGTTCGTTGTTCAGGC | |
| CV_4206NB-Fw | CACTGGATCAAGGCCGAGA | Fragment with 122 bp of the coding region |
| CV_4206NB-Rv | GAAAAACCGCTCTTCATGCTG | |
| CV_0668NB-Fw | GATCGTGGTTGCCGCTGAAA | Fragment with 121 bp of the coding region |
| CV_0668NB-Rv | CAGCACTTCCTGTTCGGTTTCC | |
| CV_1173NB-Fw | GCGTTCCAGAAATCCGTGAC | Fragment with 120 bp of the coding region |
| CV_1173NB-Rv | GATCATCTGGGTGTGGCAGG | |
| CV_3183NB-Fw | CGGGCGAAGTGATTCCGAA | Fragment with 117 bp of the coding region |
| CV_3183NB-Rv | CGCCCCAGTTGTCGCTATAG | |
| CV_3399NB-Fw | CGACCTGAAGCTGGAAATGGA | Fragment with 115 bp of the coding region |
| CV_3399NB-Rv | CTCGCTCTCCAGGATGTCTTC | |
| CV_3412NB-Fw | AATCTGTTGGCCGGCAAGAG | Fragment with 125 bp of the coding region |
| CV_3412NB-Rv | GCTTCCTTCGGCGAAATGTAGT | |
| CV_3648NB-Fw | GCCTGGACCTGATCTACGGT | Fragment with 117 bp of the coding region |
| CV_3648NB-Rv | GCAGATGGGCGTAGTTGTACA | |
| CV_0362NB-Fw | GAGCAATACCACCCAGCCTT | Fragment with 117 bp of the coding region |
| CV_0362NB-Rv | TTGTCCCAGAACACGTAGGC | |
| CV_1440NB-Fw | GAAACCTTCACCGTGGACAA | Fragment with 113 bp of the coding region |
| CV_1440NB-Rv | CCGTTATTGGGGCAAGACT | |
| CV_2001NB-Fw | GGCGAACGATCCCAATGAAA | Fragment with 119 bp of the coding region |
| CV_2001NB-Rv | TCTCGATGTAGATGCCACCG |
a
Underlined letters indicate the restriction enzyme recognition sites, used for cloning purposes.