FIG 5.
Organization and direction of transcription of the downregulated genes in the Rifr3 and Rifr5 mutants inactivated in this work and their surroundings according to the annotation of the genome of A. baumannii strain ATCC 17978. (A) RS17025 to RS17055. (B) RS14730 to RS14745. The small arrows indicate the oligonucleotides used (listed in Table S2 in the supplemental material). The large black arrows represent genes belonging to the same transcriptional unit, determined by RT-PCR analysis. The RT-PCRs were carried out in the presence of cDNA, total RNA, and DNA using the indicated combination of oligonucleotides amplifying a region of overlap between the specified genes of A. baumannii strain ATCC 17978. ϕX174 DNA/HinfI marker (Biotools) was used as a molecular size marker (lanes M). The lengths of the products amplified by the indicated oligonucleotide pairs are reported with respect to some of the marker bands.