FIG 6.
Passive transfer of immune serum provides protective immunity against challenge with a lethal dose of WT B. mallei bacteria. (A) Experimental timeline and details. BALB/c mice vaccinated intratracheally with 104 CFU of the batA KO mutant strain and back-challenged with WT B. mallei ATCC 23344 (30 days postvaccination) were used as an efficacy benchmark. (B) Kaplan-Meier survival curves. (C) Survival data during the acute and chronic phases of infection. (D, E, and F) Tissues were collected from mice that survived challenge (day 32), homogenized, diluted, and spread on agar plates to determine bacterial loads. The symbols show data for individual animals; the horizontal lines represent the mean total CFU for each group.