TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Yersinia pestis strains | ||
| 201 | Wild-type strain | 48 |
| 201 ΔyopK | yopK gene of strain 201 replaced by a kanamycin cassette using the λRed recombinant system | This study |
| 201 ΔyopK-CyopK | pACYC-yopK electroporated into 201 ΔyopK | This study |
| 201 ΔyopK-CyopKΔ91–124 | pACYC-yopKΔ91–124 electroporated into 201 ΔyopK | This study |
| 201/YopE-TEM | pBBR1-YopE-TEM electroporated into 201 | This study |
| 201/YopK-TEM | pBBR1-YopK-TEM electroporated into 201 | This study |
| 201/TEM | pBBR1-TEM electroporated into 201 | This study |
| 201 ΔyopK/YopE-TEM | pBBR1-YopE-TEM electroporated into 201 ΔyopK | This study |
| 201 ΔyopK-CyopK/YopE-TEM | pBBR1-YopE-TEM electroporated into 201 ΔyopK-CyopK | This study |
| ΔyopK-CyopKΔ91–124/YopE-TEM | pBBR1-YopE-TEM electroporated into 201 ΔyopK-CyopKΔ91–124 | This study |
| Plasmids | ||
| pET-YopK-GFP | yopK gene fused with GFP-coding sequence and cloned into pET28a | This study |
| pET-YopKΔ91–124-GFP | yopK gene lacking coding sequence of amino acids 91–124 fused to GFP gene and cloned into pET-28a | This study |
| pGEX-YopK | yopK gene cloned into pGEX-4T-2 | This study |
| pGEX-YopK1–60 | Coding sequence of amino acids 1–60 of YopK cloned into pGEX-4T-2 | This study |
| pGEX-YopK1–124 | Coding sequence of amino acids 1–124 of YopK cloned into pGEX-4T-2 | This study |
| pGEX-YopK61–124 | Coding sequence of amino acids 61–124 of YopK cloned into pGEX-4T-2 | This study |
| pGEX-YopK91–182 | Coding sequence of amino acids 91–124 of YopK cloned into pGEX-4T-2 | This study |
| pGEX-YopKΔ91–124 | yopK gene lacking coding sequence of amino acids 91–124 cloned into pGEX-4T-2 | This study |
| pACYC-yopK | Coding sequence of yopK with ∼300 bp of upstream sequence cloned into pACYC184 | This study |
| pACYC-yopKΔ91–124 | Coding sequence of yopK lacking bp 271–372 and ∼300 bp of upstream sequence cloned into to pACYC184 | This study |
| pBBR1MCS | Empty vector for construction of TEM-1 beta-lactamase reporter | Lab collection |
| pBBR1-TEM | TEM-1 beta-lactamase-coding sequence cloned into pBBR1MCS vector | This study |
| pBBR1-YopE-TEM | Coding sequence and ∼300 bp upstream sequence of yopE cloned into pBBR1-TEM directly upstream of TEM-1-coding sequence | This study |
| pBBR1-YopK-TEM | Coding sequence and ∼300 bp upstream sequence of yopK cloned into pBBR1-TEM directly upstream of TEM-1-coding sequence | This study |