Induction of cellular vacuolation by ArtB. U937 cells, HBMECs, and Vero cells were incubated with or without 10 μg/ml ArtB for 4 or 24 h, as indicated, and then examined by phase-contrast microscopy (bars = 0.1 mm [top {U937}] and 0.2 mm [middle and bottom]). Solid arrows, normal cells; hollow arrows, vacuolated cells.