FIG 7.

Colocalization of ArtB with Rab5, EEA-1, Rab9, Rab7, WGA, golgin 97, or ER-Tracker Red. Vero cells were pulsed with ArtB (10 μg/ml) for 30 min, washed twice with PBS, and incubated in fresh medium for the remainder of the indicated times. Cells were then fixed, permeabilized, and stained with anti-His₆ (to detect ArtB) and either anti-Rab5, -EEA-1, -Rab9, -Rab7, -WGA, -golgin 97, or -ER-Tracker Red, followed by fluorescent secondary antibodies, as appropriate. Cells were then examined by confocal microscopy as described in Materials and Methods. The nuclei were stained with DAPI (bar, 20 μm).