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Effect of ArtB on the cytoskeleton. Vero cells were incubated with DyLight 488-labeled ArtB (10 μg/ml) for the indicated times, after which cells were fixed and permeabilized, and F-actin was stained with 5 U/ml Alexa Flour 594-phalloidin and then examined by confocal microscopy, as described in Materials and Methods. Separate ArtB and phalloidin channels are shown, as are overlaid images with nuclei also stained with DAPI.
