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. 2017 Jun 16;24(8):1431–1442. doi: 10.1038/cdd.2016.152

Figure 2.

Figure 2

MiR-26a and -26b inhibit LECs proliferation and TGFβ2-induced EMT in vitro. (a) EdU staining analysis of LECs proliferation after transfected with miRNA negative control mimic (NC-m), miR-26a mimic (26a-m), or miR-26b mimic (26b-m), miRNA negative control inhibitor (NC-i), miR-26a inhibitor (26a-i), or miR-26b inhibitor (26b-i) for 48 h, respectively. Scale bar, 40 μm. (b) Quantification of EdU-positive cells (n=24 randomized fields per group); *P<0.05. (c) Immunofluorescent staining analysis of EMT markers FN, Col I and Col IV in LECs transfected with miRNA negative control mimic, miR-26a mimic or miR-26b mimic, and treated with TGFβ2 (5 ng/ml) for 48 h. Scale bar, 20 μm. (d) Western blot analysis of FN, α-SMA, Col IV, Snail and Slug protein levels in LECs transfected and treated as indicated in (c)