Figure 7.
SR1 enhances iTreggeneration in vitro. Human CD4+CD45RA+ were cultured with IL-2 alone, IL-2 and TGF-β, or IL-2 and TGF-β along with 0.75 μM, 1.5 μM, or 2.25 μM of SR1 (A) or with 2.25 μM or 109 nM of rapamycin (B). After 14 days of culture, cells were isolated and stained for FoxP3 expression. Flow cytometry histograms are representative images of 2 independent experiments. Graph represents pooled data from the 2 experiments; n = 4 in each group. *P < .05; **P < .001 by Student t test.