Figure 5.

Effect of 2ME2 and hypoxic conditions on the expression of TNF-α, IL-6, and IL-10 in H1N1-infected cells. Before A549 and THP-1 cells were infected for 24 h with H1N1 at MOIs of 1 and 5, respectively, they were pretreated with 0.1 and 0.2 μM 2ME2 for 30 min. A549 and THP-1 cells were exposed to 1% O₂ for 24 h after H1N1 infection. qRT-PCR was performed to measure mRNA levels of TNF-α (A), IL-6 (B) and IL-10 (C). ELISA was performed to detect the levels of TNF-α (D), IL-6 (E) and IL-10 (F) in cell supernatants. The data are shown as the mean±s.e.m. of three independent experiments. *P<0.05, **P<0.01 compared with the control group. ^#P<0.05, ^##P<0.01 compared with the H1N1-infected group