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. 2017 Apr 24;6(4):e323. doi: 10.1038/oncsis.2017.18

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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TCRP1 overexpression promotes cell transformation of NIH/3T3 cells. NIH/3T3 cells were transfected with TCRP1-overexpressing vector, (a) TCRP1 expression was analyzed by using the western blot method. β-Actin was used as an inner control. (b) Cell viability was measured by MTS assay. (c, d) Clonogenic capacity was measured by plate cloning assay (c) and soft agar cloning assay (d). (e) Cells were fixed in ethanol, and stained with propidium iodide, and then DNA contents were determined by flow cytometry. The percentage of cells with G1, S and G2 was calculated using Flowjo analysis software. Experiments were repeated three times. *P <0.05.