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. 2017 Apr 10;6(4):e312. doi: 10.1038/oncsis.2017.21

Figure 4.

Figure 4

FBP1 is a key mediator of TRIM28-regulated HCC growth in culture and in mice. (a, b) SK-Hep-1 cells were infected with lentivirus expressing control or TRIM28 and/or FBP1-specific shRNAs. Cells were harvested for western blot analysis (a) and MTS assay (b) after 48-h infection. All data show mean values±s.d. (error bar) (n=6). *P< 0.01. (c, d) SK-Hep-1 cells were infected with lentivirus as in a. Seventy-two hours after infection, cells were administered into subcutaneous layer of the right flank of mice. Xenografts growth was measured every other day for 21 days, and tumors were harvested and photographed. Data present means + s.d. (n=4). *P<0.01. (e) Tumors harvested in c were sectioned for hematoxylin and eosin (H&E) and IHC of TRIM28 and FBP1 in xenografts tissues as indicated. (f) IHC analysis of Ki-67 expression in xenografts was performed and staining was quantified. All results shown are mean values± s.d. (error bar) (n=4). *P<0.01.