Figure 6.

Bortezomib inhibits FBP1 degradation and regulates Warburg effect in HCC. (a-c) HepG2 cells were transfected with indicated constructs for 20 h followed by treatment with 80 nM of bortezomib for 8 h. Cells were then harvested for western blot analysis (a) and reverse transcriptase (RT)–quantitative PCR (qPCR) (b) and spent medium were used for measurement of glucose and lactate levels (c). *P< 0.01; NS, not significant. (d, e) HepG2 and SK-Hep-1 cells were infected with lentivirus-expressing control or FBP1-specific shRNAs. Twenty-four hours after infection, cells were treated with 40 nM of bortezomib for another 24 h, and the cells were harvested for western blot analysis (d) and spent medium was collected for measurement of glucose and l-lactate levels (e). *P< 0.01. (f) HepG2 and SK-Hep-1 cells were infected with lentivirus expressing control or FBP1-specific shRNAs. Forty-eight hours after infection, cells were treated with 20 nM of bortezomib for MTS assay. All data are mean values±s.d. (error bar) (n=6). *P <0.01; **P<0.001.