Table 1. Virological characteristics of patients with chronic hepatitis B who provided HBV inocula.

Inoculum	Patient age (years)/sex	HBV DNA(IU/ml)a	HBV genotypeb	HBsAgc	HBeAgc	Anti-HBec	Anti-HBcc	HIV RNAd	Anti-HCVe
NL01	58/M	2.7 × 107	A	pos	pos	neg	pos	neg	neg
NL02	35/F	1.1 × 108	C	pos	n.a.	n.a.	pos	neg	neg
NL03	33/M	5.8 × 108	E	pos	pos	n.a.	pos	neg	neg

Abbreviations: anti-HBc, antibodies to HBV core antigen; anti-HBe, antibodies to HBeAg; anti-HCV, antibodies to hepatitis C virus; F, female; HBeAg, HBV e antigen; HBsAg, HBV surface antigen; IU, international units; M, Male; n.a., not available.

^aDetermined by COBAS Ampliprep/TaqMan HBV Test (sensitivity 20 IU/ml) Roche Diagnostics, Laval, Quebec, Canada.

^bAssayed by in-house PCR with HBV S gene-specific primers (forward primer: 5′-GCCTCATTTTGTGGGTCACCATA-3' and reverse primer: 5′-ATAACTGAAAGCCAAACAGTGGG-3') and bidirectional sequencing of the resulting 1115-bp amplicons.

^cDetermined by ARCHITECT immunoassays from Abbott Diagnostics, Mississauga, Ontario, Canada.

^dTested by Abbott Real Time HIV-1 assay m2000 (sensitivity 75–10⁷ virus copies/ml), Abbot Diagnostics, Mississauga, Ontario, Canada.

^eDetermined by COBAS Amplicor HCV v2.0 (sensitivity 50–60 IU/ml), Roche Diagnostics, Laval, Quebec, Canada.