Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 17;6(4):e320. doi: 10.1038/oncsis.2017.5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

p16 loss promotes transformation and tumorigenesis of RB1-deficient lung epithelial cells. (a, b) Representative low (a) and high (b) power images of subcutaneous tumors arising after injection of Rb1/p16-deficient lung epithelial cells into three independent mice showing histologic features of pleomorphic non-small cell lung cancer (NSCLC) including malignant giant cells with markedly enlarged nuclei (arrows) and spindled cells (arrowheads) invading host adipose tissue (ad) and skeletal muscle (sm). (c–j) Representative lung tumors and metastases in Rb1^Δ/Δp16^+/− and Rb1^Δ/Δp16^−/− mice. Pleomorphic NSCLC (c) with metastasis to chest wall (d, e) and histologic features similar to subcutaneous tumors, including giant cells with enlarged nuclei (arrows) and spindled cells (arrowheads) invading skeletal muscle (sm). Gross image of small cell lung cancer (SCLC) (f, t) replacing right lower lung lobe with adjacent normal middle lung lobe (lu) and mediastinal metastasis (met). Multifocal non-small cell lung (NSCL) tumors with extensive airway invasion (g–h, t) and a second mouse with multifocal NSCL tumors (i, t) including adenocarcinoma metastasis (j, met) to mediastinal lymph node (j, ln). (k–n) Representative lung tumors in Rb1^Δ/Δp16^+/+ mice. One or two tumors (k, t) per mouse were present with papillary and/or solid growth (l) lacking the marked cytological atypia seen in Rb1/p16-deficient tumors (m) and only focal invasion of airway (n). (o) Small cell lung (SCL) and NSCL tumors (t) in a single Rb1^Δ/Δp16^+/− mouse showing characteristic morphology and immunostaining for CALCA but not SCGB1A1 and SFTPC in SCL tumor and SFTPC but not SCGB1A1 and CALCA in NSCL tumor (representative of 4 SCL and 16 NSCL tumors including 4 metastatic tumors). Original magnifications: × 2 (g, i, k); × 10 (h and o left column); × 20 (a, c, j, l, n); × 40 (O right 4 columns); × 100x (b, d, e, m).