Table 3. Upregulated serum miRNAs evaluated by qRT–PCR in the peripheral blood of patients with gastric cancer when compared with healthy controls.

miRNAs	ΔCthealthy	ΔCtcancer	ΔΔCt	2–ΔΔCt	Corrected Pa
miR-518f	−4.50±10.78	−12.35±1.99	−7.85	230.67	0.017
miR-130a	10.96±3.48	6.62±3.42	−4.34	20.22	0.017
miR-212	8.81±2.36	4.58±2.65	−4.22	18.70	0.007
miR-220	7.85±2.51	3.85±2.81	−4.00	15.98	0.017
miR-433	9.03±1.67	5.19±3.15	−3.84	14.29	0.025
miR-518d	8.77±3.76	5.43±3.24	−3.35	10.18	0.037
miR-331	6.54±2.61	3.31±3.34	−3.23	9.40	0.039
miR-19a	8.39±4.07	5.18±3.55	−3.21	9.23	0.042
miR-365	10.66±3.22	7.62±3.12	−3.04	8.25	0.037
miR-374	8.48±3.24	5.55±2.94	−2.93	7.62	0.037
miR-296	7.74±4.29	4.86±3.14	−2.88	7.37	0.039
miR-323-3p	9.21±1.60	6.59±2.94	−2.63	6.18	0.046
miR-21	4.05±2.33	1.73±2.45	−2.33	5.02	0.017
miR-146b	6.95±2.34	4.64±2.21	−2.31	4.97	0.037
miR-146a	5.99±2.19	3.68±2.65	−2.31	4.96	0.025
miR-106a	4.30±2.77	2.10±2.51	−2.19	4.57	0.025
miR-223	−2.65±2.32	−4.78±2.57	−2.12	4.35	0.025
miR-19b	2.45±2.23	0.36±2.63	−2.09	4.26	0.025
miR-451	0.68±2.31	−1.41±2.84	−2.08	4.23	0.037
miR-30c	4.95±3.3	2.90±2.65	−2.05	4.14	0.037

Abbreviations: miRNA=microRNA; qRT–PCR=quantitative reverse transcriptase PCR.

Values are expressed as means±s.d.

ΔC_t=C_{t sample}−C_{t average expression of cel-miR-39 and miR-191-5p.}

ΔΔC_t=ΔC_{t cancer}−ΔC_{t healthy.}

^aBenjamini, Krieger, and Yekutieli correction for multiple testing.