Table 1. The influence of MHC genotypic combination of mates on male sperm velocity in ovarian fluid in Chinook salmon.
| Estimate | Variance (%) | Lower 95% CI | Upper 95% CI | pMCMC | |
|---|---|---|---|---|---|
| Fixed effects | |||||
| Intercept | 59.58 | 41.08 | 74.73 | ||
| AAI | 0 | −0.016 | 0.014 | 0.952 | |
| Season | 21.37 | 6.190 | 35.612 | 0.008 | |
| Random effects | |||||
| Female (10) | 47.05 | 8.03 | <0.001 | 212.770 | |
| Male (27) | 133.95 | 22.86 | <0.001 | 269.263 | |
| Female–Male (57) | 266.25 | 45.42 | 113.135 | 427.263 | |
| Residual | 138.93 | 23.70 | 99.881 | 184.834 | |
| Summary statistics | |||||
| Marginal R2 | 0.13 | ||||
| Conditional R2 | 0.80 | ||||
| Fixed effects | |||||
| Intercept | 56.13 | 37.867 | 72.534 | ||
| AAII/PBRII | 29.39 | −101.089 | 150.775 | 0.662 | |
| Season | 21.49 | 6.327 | 38.960 | 0.012 | |
| Random effects | |||||
| Female (10) | 52.99 | 9.08 | <0.001 | 205.096 | |
| Male (27) | 131.03 | 22.45 | <0.001 | 278.514 | |
| Female–Male (57) | 259.28 | 44.43 | 107.408 | 413.900 | |
| Residual | 140.29 | 24.04 | 101.970 | 186.994 | |
| Summary statistics | |||||
| Marginal R2 | 0.13 | ||||
| Conditional R2 | 0.80 | ||||
| Fixed effects | |||||
| Intercept | 62.67 | 40.074 | 81.172 | ||
| PBRI | 0.00 | −0.012 | 0.006 | 0.52 | |
| Season | 22.00 | 5.298 | 36.864 | 0.008 | |
| Random effects | |||||
| Female (10) | 66.89 | 11.48 | <0.001 | 240.959 | |
| Male (27) | 123.15 | 21.14 | <0.001 | 263.199 | |
| Female–Male (57) | 253.21 | 43.47 | 113.123 | 419.656 | |
| Residual | 139.15 | 23.91 | 97.164 | 188.349 | |
| Summary statistics | |||||
| Marginal R2 | 0.13 | ||||
| Conditional R2 | 0.79 | ||||
| Fixed effects | |||||
| Intercept | 58.68 | 46.396 | 72.988 | ||
| MxyI | 2.26 | −9.320 | 14.469 | 0.68 | |
| Season | 21.08 | 5.536 | 35.962 | 0.014 | |
| Random effects | |||||
| Female (10) | 45.11 | 7.82 | <0.001 | 215.784 | |
| Male (27) | 129.94 | 22.53 | <0.001 | 280.324 | |
| Female–Male (57) | 264.46 | 45.86 | 95.492 | 432.000 | |
| Residual | 137.18 | 23.79 | 98.802 | 183.879 | |
| Summary statistics | |||||
| Marginal R2 | 0.13 | ||||
| Conditional R2 | 0.80 | ||||
| Fixed effects | |||||
| Intercept | 65.339 | 51.112 | 81.668 | ||
| MxyII | −5.18 | −11.660 | 1.641 | 0.136 | |
| Season | 22.77 | 5.433 | 37.791 | 0.01 | |
| Random effects | |||||
| Female (10) | 57.04 | 9.95 | <0.001 | 231.801 | |
| Male (27) | 137.89 | 24.07 | 24.964 | 276.052 | |
| Female–Male (57) | 241.61 | 42.28 | 95.605 | 393.166 | |
| Residual | 136.30 | 23.79 | 97.052 | 181.299 | |
| Summary statistics | |||||
| Marginal R2 | 0.13 | ||||
| Conditional R2 | 0.80 | ||||
Abbreviations: CI, confidence interval; MHC, major histocompatibility complex; PBR, peptide-binding region.
MCMCglmm analyses yielded significant pMCMC values (in bold) of fixed effects (season, MHC divergence). The amount of variation explained by fixed effects (marginal R2) and by the whole model (conditional R2) are reported. The variance (%) explained by each random effect refers to the total variance by all random effects (female identity (n=10), male identity (n=27), female–male interaction (n=58) and the residual variance. Following estimates of divergence between mates at MHC class I and II were tested for an impact on sperm velocity in ovarian fluid: i) divergence at the peptide sequence at the whole exon (denoted AAI and AAII), ii) divergence at the putative sites involved in peptide binding only (PBRI and PBRII) and iii) number of MHC alleles shared between mates (MxyI and MxyII). As all polymorphic sites at MHC class I are part of the PBR, divergence at the whole exon and the PBR are identical and thus, not listed separately.