Table 3. Estimates of effective population size (N _e) for each population and cluster of Calotriton arnoldi and for the four Calotriton asper populations, calculated with three programs: LDNe, ONeSAMP, and COLONY; estimations of the upper and lower 95% CI estimates for each method are indicated.

		LDNe			OneSamp			COLONY			Relationship
		Ne	95% CIs		Ne	95% CIs		Ne	95% CIs		Unrelated	Half siblings	Full siblings	Parent offspring
C. arnoldi	Population
	A1	342.30	77.00	Infinite	27.65	24.51	34.81	46.00	26.00	90.00	91.70	7.51	0.40	0.40
	A2	49.40	32.20	93.20	33.94	29.94	41.89	40.00	25.00	71.00	90.31	7.12	0.85	1.71
	A3	142.10	61.80	Infinite	36.85	33.33	43.20	44.00	28.00	80.00	91.17	7.98	0.28	0.57
	B1	55.80	34.10	126.40	31.59	27.77	40.69	35.00	20.00	68.00	86.33	12.00	0.67	1.00
	B2	62.20	27.50	15091.10	36.39	30.46	53.44	31.00	18.00	57.00	81.75	13.23	1.85	3.17
	B3	7.30	2.40	21.70	14.97	12.61	19.61	13.00	7.00	30.00	68.31	8.00	12.31	11.38
	B4	Infinite	Infinite	Infinite	5.54	4.87	6.64	—	—	—	100	—	—	—
	Clusters
	A1–A2	44.50	36.00	56.50	85.95	66.52	127.10	60.00	41.00	92.00	84.16	14.37	0.57	0.90
	B1–B2–B4	30.00	23.60	39.00	80.14	55.44	157.01	42.00	27.00	66.00	80.89	15.91	1.50	1.69
C. asper	Population
	Ibón de Perramó	349.80	100.30	Infinite	42.80	33.96	62.07	40.00	26.00	65.00	84.13	12.68	1.60	1.60
	B. de Valdragás	1293.00	201.40	Infinite	41.41	35.29	58.22	58.00	37.00	98.00	90.69	8.50	0.40	0.40
	Ibón de Acherito	172.80	89.50	1078.90	60.80	46.42	99.78	68.00	44.00	111.00	88.85	9.62	0.51	1.03
	Bassies	92.00	61.90	149.50	28.73	21.65	41.06	72.00	53.00	100.00	78.02	16.10	3.57	2.31

Abbreviation: CI, confidence interval. Relationship indicates the percentage of individual relatedness within each population and cluster.