Figure 1. Representative replication inhibition and cytotoxicity experiments.

Replication inhibition by HIDs and an HPD was measured against an HBV genotype D isolate in HepDES19 cells and plotted against compound concentration (log₁₀[M]). Cytotoxicity was assessed by MTS, NR, and LDH assays and plotted against compound concentration (log₁₀[M]). EC₅₀ experiments were done with a single replicate per condition and CC₅₀ assays were each conducted in triplicate. EC₅₀ values were calculated based on the decline of the plus-polarity DNA strand; positive-polarity DNAs are in black; negative-polarity DNAs are in grey. EC₅₀ and CC₅₀ values are reported as the average of two or three independent experiments ± one standard deviation.