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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Antiviral Res. 2017 Apr 25;143:205–217. doi: 10.1016/j.antiviral.2017.04.012

Figure 4. Detection of capsid-associated RNA:DNA heteroduplexes.

Figure 4

HepDES19 cells replicating HBV were treated with 4X EC50 of the HID compound #89, 30 μM of the inactive compound #138, or with DMSO as a vehicle control. HBV capsids were harvested and encapsidated nucleic acids were purified. The nucleic acids were split into two pools, one that was mock treated and the other which was treated with RNaseA. Nucleic acids were resolved by electrophoresis and HBV sequences were detected by Southern analysis. RNA:DNA heteroduplex accumulation was determined as a collapse in migration patterns in the RNaseA-treated samples. rcDNA, relaxed circular DNA; DS DNA, double-stranded DNAs; ssDNA, single-stranded DNAs.