Sampling locations for B. tricaudatus (black), B. bicaudatus (white) or both species (black center and white outline). Sampling spanned three drainages in the Colorado Rockies (represented by different shapes, circles: Poudre [P], squares: Big Thompson [B], triangles: St. Vrain [S]). Site IDs indicate drainage (P, B or S) followed by elevation in meters. The star in the inset figure shows the sampling location in North America. Axes represent longitude and latitude, and the map is oriented with north at the top.