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. Author manuscript; available in PMC: 2017 Jul 21.
Published in final edited form as: Curr Opin Solid State Mater Sci. 2014 Sep 19;18(6):308–318. doi: 10.1016/j.cossms.2014.07.004

Fig. 2.

Fig. 2

Glial and neuronal alignment in engineered 3-D microsystems In Vitro. Neuronal-astrocytic co-cultures formed in 3-D around engineered micro-towers (250 μm tall, 200 μm diameter). (A) Across the top 200 μm of these cultures, neural cells expressing green fluorescent protein (GFP) and astrocytic processes (glial-fibrillary acidic protein; red) coalesced into discrete tracts spanning the cellular populations directly adhered to the micro-towers. (B) In this system, neurons (GFP+) grew in alignment with long astrocytic processes (GFAP+; red) spanning the micro-towers. Adapted from [43].