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. 2017 May 25;8(5):e2821. doi: 10.1038/cddis.2016.216

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RGS2 is critical for β-cell apoptosis in vivo. (a) Absorbance at OD405 in RGS2^+/+ and RGS2^−/− islets 24 h after hypoxia challenge (measured by the Cell Death Kit according to the manufacturer’s recommendation). (b) Protein expression of cleaved Caspase-3 and β-actin analyzed in isolated islets harvested from RGS2^+/+ and RGS2^−/− mice after exposing to hypoxia for 6 h. (c) Transcriptional changes in expression of apoptosis-related genes 6 h after hypoxia challenge in islets from RGS2^+/+ and RGS2^−/− mice (n=3–4). (d) Cell death in pancreas from RGS2^+/+ and RGS2^−/− mice (TUNEL assay). Arrow points to TUNEL⁺ cell. Scale bar=50 μM. (e) Percentage of TUNEL⁺ cells/Insulin⁺ cells (n=3 or 4 per group). *P<0.05, **P<0.01, Student's t-test