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. 2017 May 4;8(5):e2759. doi: 10.1038/cddis.2016.464

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PEG3 regulates apoptosis in TCam-2 cells. (a) Detection of PEG3 protein expression in cells transfected with short hairpin RNA against PEG3 (shPEG3-1 or shPEG3-2) or vector control (shControl) by western blot analysis. (b and c) Evaluation of the effect of PEG3 silencing on apoptosis using caspase-3 activity (n=8; b) and cleaved PARP (n=3; c) assays. (d) Western blot analysis of cleaved PARP in cells co-transfected with miR-514a-3p and PEG3 full-length coding sequence without 3’ UTR (CDS) or vector control. GAPDH was used as a loading control. Error bars represent S.D. of the mean of at least three independent experiments. Differences between two groups were evaluated using paired t-test and P<0.05 as significant