Figure 5.

Silencing of PEG3 and miR-514a-3p overexpression suppress NF-κB reporter activity. (a) Evaluation of NF-κB luciferase activity in TCam-2 cells co-transfected with NF-κB reporter vector and shPEG3-1 or miR-514a-3p mimic, as compared with their respective negative controls. (b) IP was performed on TCam-2 cells expressing Myc-PEG3 or vector control using anti-Myc or -IgG. The immunoprecipitates and input (2%) were analyzed by immunoblotting using anti-TRAF2 antibody. Low and high refers to the same western blot detected at low and high exposure time, respectively. Error bars indicate S.D. of the mean of three independent experiments. (c) Model of PEG3-mediated NF-κB activation in TGCT, in which increased expression of PEG3 protect cells from apoptosis by activation of NF-κB