Figure 2.

Procedural separation of cell death and thermal allodynia. (a) Epidermal morphology changes and TUNEL staining with different doses of UV, 24 h post irradiation. Anti-Fasciclin-3 (membranes, green) and TUNEL labeling (apoptotic cells, red). Bar, 50 μm. (b) Dependence of UV-induced thermal allodynia upon various doses of UV, 24 h post irradiation. (c) Presence of TUNEL labeling in responsive or non-responsive larvae. n=9–12 in each group. (d) Epidermal morphology changes and TUNEL staining of responders and non-responders 24 h after 17 mJ/cm² UV irradiation. Anti-Fasciclin-3 (membranes, green) and TUNEL labeling (apoptotic cells, red). (e) UV-induced thermal allodynia when dronc (initiator caspase) and eiger (TNF) were inhibited. UAS-RNAi lines are indicated