Figure 5.

miR-130a-3p inhibited cell proliferation and growth, and induced apoptosis in activated HSCs. (a) The miR-130a-3p mimics regulated the expression of miR-130a-3p. qRT-PCR validated the expression of miR-130a-3p after transfection with the miR-130a-3p mimics. The miR-130a-3p mimics significantly upregulated the expression of miR-130a-3p. Values represent the mean±S.D., ***P<0.001 compared with control. (b) miR-130a-3p expression was examined by real-time qRT-PCR at 0, 24, 48, 72 h after transfection with the miR-130a-3p mimics. Values represent the mean±S.D. (**P<0.01). (c) miR-130a-3p inhibited HSC-T6 cell growth as determined by an MTT assay. Values represent the mean±S.D., **P<0.01, ***P<0.001 compared with control. (d) Annexin V/PI combined with flow cytometry. (e) The mRNA expression of caspase-3, caspase-9, and PARP1 and (f) the protein expression of cleaved caspase-3, caspase-9, and PARP1 were increased after transfection with the miR-130a-3p mimics. β-actin was used as a loading control. Values represent the mean±S.D., ***P<0.001 compared with control