Figure 8.

Overexpression of TGFBR1 and TGFBR2 rescued miR-130a-3p-impaired proliferation and cell growth in HSCs. The HSC-T6 cells were transduced with the miR-130a-3p mimics and the TGFBR1-expressing and TGFBR2-expressing vectors. The rescue efficiency of TGFBR1 or TGFBR2 in the HSC-T6 cells was confirmed by (a) RT-PCR and (b) western blotting. β-actin was used as a loading control. Values represent the mean±S.D. (***P<0.001). Overexpression of TGFBR1 and TGFBR2 rescued the (c) mRNA and (d) protein expression of Smad2 and Smad3. β-actin was used as a loading control. Values represent the mean±S.D. (*P<0.05, **P<0.01, ***P<0.001). Overexpression of TGFBR1 and TGFBR2 rescued the (e) mRNA and (f) protein expression of Col-1 and Col-4. β-actin was used as a loading control. Values represent the mean±S.D. (*P<0.05, **P<0.01, ***P<0.001). (g) A schematic diagram highlighting the regulation of HSCs by miR-130a-3p during the genesis and resolution of liver fibrosis