Figure 4.

CCL5 activates Slug in colon cancer cells. (a) After Scrambled or Slug siRNA treatment, the SW1116 cells were exposed to TCM or 20 ng/ml for 24 h. The expression of Slug was determined by real-time PCR analysis in SW1116. Data are presented as the means±S.D. n=3. *P<0.05, ***P<0.001; (b) The expression of Slug was determined by western blot analysis in SW1116 treated with TCM or 20 ng/ml CCL5 for 24 h. Data are presented as the means±S.D. n=3. **P<0.01, ***P<0.001. Representative blot was shown below; (c) After 24 hours of Scrambled or Slug siRNA treatment, SW1116 cells were seeded in the upper chamber and examined their migratory capability to TCM or CCL5. Quantification was presented as mean±S.D. from three independent experiments. *P<0.05; **P<0.01; (d) After 24 h of Scrambled or ccl5 siRNA treatment, hMSCs were treated with 10 ng/ml TNF-α for 24 h and collected for TCM. SW1116 cells were treated with TCM for 24 h and examined for Slug expression by real-time PCR. Data are presented as the means±S.D. n=3. *P<0.05, ***P<0.001