Figure 4.

IRF2 functions to suppress NSCLC. (a) H23, H1299 and A549 cells were transfected with siNC or siIRF2, and cell proliferation was determined by CCK-8. (b) The apoptosis distributions of H23, H1299 and A549 cells were transfected with siNC or siIRF2 for 48 h then detected by flow cytometry. (c, d) 48 h after transfected with siNC or siIRF2, the results of wound healing assays and Transwell assays in H23, H1299 and A549 cells. (e, f). H1299 and A549 cells were co-transfected with miR-18a-5p mimic and pcDNA3.1/pcDNA3.1-IRF2 vector then cell migration ability subject to Transwell migration and invasion assays, and cell apoptosis was detected 48 h later by flow cytometry analyses. n=3–4 independent experiments, error bars represent the mean±S.E.M. *P<0.05, **P<0.01, ***P<0.001