Figure 1.

miR-218-5p is downregulated in GBC tissues and cell lines, and is associated with prognosis of GBC patients. (a) Volcano plot of microRNAs expression array from six pairs of GBC and CNG tissues. More detailed information can be found in Supplementary Materials. (b) miR-218-5p expression in other nine types of cancer from starBase v2.0 Pan-cancer Project database. (c) Validation of miR-218-5p differential expression in an independent cohort contained 36 pairs of GBC and CNG tissues. (d) Fold change of miR-218-5p expression in new 36 pairs of GBC and CNG tissues. (e) Comparison of miR-218-5p expression in 82 pairs of GBC and CNG FFPE tissues (left) and their representative ISH image stained by anti-miR-218-5p probe (right). (f) Relative expression of miR-218-5p in GBC cells and GBE cells. (g) Comparing different TNM stages, tumor size, CA19-9 level, gallstone status, age and gender ratio between miR-218-5p high and miR-218-5p low expression from 82 pairs of GBC FFPE tissues. Statistical significance was performed by the χ²-test. (h) Kaplan–Meier analysis of the correlation between miR-218-5p expression and POS in 82 GBC patients. Small nuclear RNA U6 was used to normalize the Q-PCR results. bar, S.E.M., **P<0.01; ***P<0.001; Student’s t-test. BLCA, urothelial bladder cancer; BRCA, breast cancer; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KIRC, clear cell kidney carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; SKCM, cutaneous melanoma; THCA, papillary thyroid carcinoma