Figure 3.

(a) C-33A cells endogenously express Cx32, unlike HeLa or SiHa cells (n=3). (b) The endogenous GJIC of C-33A cells was abolished by gap junction inhibitors 2APB and 18α-GA (n=3). (c) Western blot comparing effectiveness of three siRNAs in reducing expression of Cx32 in C-33A cells. (n=3). (d) Suppression of SN-induced apoptosis by treatment with 2APB or 18α-GA in C-33A cells (n=7). (e) The anti-apoptotic effect of 2APB and 18α-GA in C-33A cells was reversed by Cx32 RNA interference (n=5). Error bar: standard error. *P<0.05; n=1 represents an independent cell culture