Figure 4.

Transplantation of lentivirus-transduced BMMSCs ameliorated brain damage indicated by the reduction of brain edema, serum S100B levels, pyknotic cells in H&E staining, and TUNEL-positive cells. (a) Changes in brain water content were measured by weighting from five animals in each group (N=5); (b) Levels of serum S100B from each animal were determined by ELISA; (c and d) Representative micrographs from H&E stained (c) or TUNEL-labeled (d) coronal sections show the differential brain damage in both hippocampal CA1 and temporal cortex areas of rat brains from respective groups. Arrows indicate pyknotic/eosinophilic cells (c) and TUNEL-positive cells (d) in both hippocampal CA1 and temporal cortex areas in coronal sections from rats with or without CA-GCII following the different treatments. Quantifications of damaged cells are depicted as percentage versus total number of cells within the respective microscopic fields (mean±S.D.). ** indicates P-value <0.001. Sham, normal control; Blank, CA-GCII with PBS; BM-Vehicle, CA-GCII with naïve BMMSCs; BM-B&V, CA-GCII with BMMSCs co-transduced with BDNF- and VEGF-lentiviruses