Figure 3.

MK2206 enhanced the induction of apoptosis by bufalin in primary myeloma cells. (a) Patients’ mononuclear cells were separated by Ficoll–Hipaque density sedimentation and CD138-positive cells were isolated and treated with 12 nM of bufalin alone and/or in addition of 6 μM of MK2206 for 48 h. The survival rates were assessed by Annexin V/PI staining. (b) Freshly isolated PBMCs from 3 healthy donors were cultured with 12 nM of bufalin and 6 μM of MK2206 for 48 h. The viability was assessed by the tryphan blue assay. Each bar represented the mean±S.E. of triplicate experiments (*P<0.05; **P<0.01)