Figure 2.

Effect of CHCHD4 reduction on cell death at 6 and 24 h after HI. (a) Representative Fluoro-Jade staining in the cortex (left) at 6 and 24 h after HI. The bar graph (right) shows the quantification of the number of Fluoro-Jade-positive dying cells. (b) Representative photomicrographs of AIF immunostaining in the cortex (left) at 6 and 24 h after HI. The bar graph (right) shows the number of AIF-positive cells (nuclear AIF immunostaining). (c) Representative western blotting of fodrin (top panel) and caspase-3 (middle panel) in both the ipsilateral (IL) and contralateral (CL) hemispheres at 24 h after HI in Wt and Het brains. Caspase-3 activation in the ipsilateral hemisphere is reflected by the appearance of a 17 kDa cleavage product in addition to the full-length 31 kDa protein (middle panel). Actin (bottom panel) was used as the loading control. Quantification of the cleaved bands did not reveal any significant difference between Wt and Het mice. (d) The bar graph shows caspase-3-like (DEVDase) activity in normal controls as well as ipsilateral hemispheres at 6 and 24 h after HI. There was no difference between Wt and Het brains in control or HI brains. *P<0.05