Figure 6.

Irf8 induction drives the monocytic/dendritic differentiation of APL progenitors. (a–b) Mice repopulated with Neo-3G or Irf8-3G APL cells (a) and with NC-3G or shIrf8-3G APL cells (b) were treated with Dox for 3 days in vivo. The percentages of CD11c⁺ and Gr-1⁺ cells within the leukemic cell compartment were analyzed by flow cytometry. The results of statistical analysis are shown in the bottom panels. (c) Quantitative RT-PCR assay on the stemness-related gene c-Myb in BM APL cells after Irf8 overexpression or knockdown. (d–e) Quantitative RT-PCR assay on the monocytic (empty box) and granulocytic (filled box) differentiation-related genes in BM APL cells after Irf8 overexpression (d) or knockdown (e). (f) Morphological inspection of Irf8-3G APL cells after Irf8 induction (left panel) or knockdown in vivo (right panel). All data in this figure are presented as the mean±S.D., *P<0.05, **P<0.01