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. 2017 May 18;8(5):e2800. doi: 10.1038/cddis.2017.204

Figure 6.

Figure 6

Overexpression of RACK1R36D/K38E also induces autophagy. HT1080 (a), HeLa (b), HepG2 and Hep3B (c) cells were transfected with pcDNA3-FLAG-RACK and RACK1R36D/K38E. After 2 days, the levels of various proteins were assessed by immunoblot analysis. (d) pcDNA-FLAG-RACK1 and pcDNA-FLAG-RACK1R36D/K38E were transfected into EGFP-LC3 stably expressing HT1080, HepG2 and Hep3B cells, and images were obtained by florescence microscopy