Figure 4.

Progressive locomotor abnormalities in Dicer^CKO mice. (a) Mean latency to fall in a constant speed rotarod assay at 15, 25, 35 and 44 r.p.m. at indicated times after start of Tam injections in control and Dicer^CKO mice (n=7 and 9, respectively). Automated threshold determination for each speed was followed by a log-rank test to calculate the indicated P values. (b and c) Quantification (b) and representative animal tracks (c) of the total distance traveled by control or Dicer^CKO mice during 5-min open field tests performed 10 weeks after start of Tam injections (n=4 and 3, respectively). *P<0.05 as determined by Student’s unpaired two-tailed t-test. (d) Injection of L-DOPA attenuated locomotor abnormalities in Dicer^CKO mice at 17 weeks after start of Tam injections measured by a constant speed rotarod assay at 15, 25, 35 and 44  r.p.m. (n=5 and 4 for control and Dicer^CKO mice, respectively). The indicated P-value was calculated by a two-way repeated measurements ANOVA