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. 2017 May 18;8(5):e2804. doi: 10.1038/cddis.2017.216

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ST7L mediates the effects of miR-23b on proliferation, colony formation and metastasis of HCC cells in vitro. SMMC-7721 cells were transiently transfected with miR-23b mimics, ST7L expression plasmids, NC mimics or empty vector, whereas HCCLM3 cells were transfected with miR-23b inhibitor, ST7L siRNA or the corresponding NC oligonucleotides. (a and b) CCK-8 assays, (c and d) colony formation assays, (e and f) wound-healing assays and (g and h) transwell invasion assays were performed. The percentage of wound closure and the number of cells passed through the membrane were counted and compared in the right diagrams. Scale bars represent 500 μm (e and f) and 200 μm (g and h). Results were represented as mean±S.D. (n=3). *P<0.05, **P<0.01