Figure 6.

Effects of miR-23b and ST7L on AKT/GSK3β/β-catenin pathway. SMMC-7721 cells were transiently transfected with miR-23b mimics, ST7L expression plasmids, NC mimics or empty vector, whereas HCCLM3 cells were transfected with miR-23b inhibitor, ST7L siRNA or the corresponding NC oligonucleotides. (a and b) Western blot analysis of ST7L, AKT (pan), p-AKT (Ser473), p-AKT (Thr308), p-GSK3β (Ser9), GSK3β, p-β-catenin (Ser33/37) and β-catenin expression in SMMC-7721 cells or HCCLM3 cells transfected with corresponding plasmids and oligonucleotides. (c) Reporter gene analysis of β-catenin transactivity in SMMC-7721 cells transfected with corresponding plasmids and oligonucleotides. Data were normalized to the activity of β-catenin in SMMC-7721 cells with NC mimics transfection. (d and e) QRT-PCR analysis of C-myc, Vimentin and Cyr61 expression in SMMC-7721 cells or HCCLM3 cells transfected with corresponding plasmids and oligonucleotides. β-Actin was used as a reference gene. (f and g) Western blot analysis of C-myc, Vimentin and Cyr61 in SMMC-7721 cells or HCCLM3 cells transfected with corresponding plasmids and oligonucleotides. *P<0.05, **P<0.01