Figure 7.

Synergistic effect induced by combination treatment was reversed by the overexpression of FOXM1 or BICD2 in NCI-H460 cells. (a, b) NCI-H460 cells were treated with 10 μM DT-13 and indicated concentration of NVB for 48 h after transfecting FOXM1 or BICD2 plasmid, and cell viability was determined by MTT assay. (c, d) NCI-H460 cells were treated with 10 μM DT-13 and 0.01 μM NVB for 12 h after transfecting FOXM1 or BICD2 plasmid, and the extent of mitotic arrest induced by combination treatment was determined by flow cytometry. (e, f) Percentages of cells arrested in G2/M phase were shown in the histograms. (g, h) Expression of MPM2, cyclin B1, cdc2 and phosphorylation of cdc2 (Tyr15) was determined by western blotting analysis, β-actin was served as the loading control. The data were expressed as mean±S.D. in triplicate using Student’s t-test (two-tailed). *P<0.05 and **P<0.01