Figure 2.

MiR-101-3p prevents serum deprivation-induced apoptosis. HUVECs were transfected with control miRNA (C-miR) or miRNA-101-3p mimic (miR-101) and cultured in serum-free or 5% FBS-supplemented media for 24 h (TUNEL and FACS analysis) or 30 h (cell viability). (a and b) Cell viability was evaluated by microscopy (a) and MTT assay (b). Scale bars, 50 μm. A: 5% FBS, B: serum-free, C: serum-free+100 nM C-miR, D: serum-free+50 nM miR-101, and E: serum-free+100 nM miR-101. (c) Apoptosis was determined by TUNEL assay, and nuclei were also stained with DAPI. Scale bars, 50 μm. A: 5% FBS, B: serum-free, C: serum-free+C-miR, D: serum-free+100 nM miR-101. (d) Quantitation of TUNEL-positive cells. (e) Cells were stained with Annexin V and PI and analyzed by FACS. A: 5% FBS, B: serum-free, C: serum-free+100 nM C-miR, D: serum-free+100 nM miR-101. (f) Quantitation of cells stained with both Annexin V and PI. *P<0.05 and **P<0.01 versus cells transfected with C-miR in serum-free media