Figure 1.
Furanodienone inhibits cells proliferation and induces G0/G1 arrest in human colorectal cancer cells. (a) The antitumor effect of furanodienone on RKO and HT-29 cells was measured by CCK-8 assay. Cells were treated with raising doses of furanodienone for 24, 48 and 72 h. Each experiment performed in triplicate. (b) Colony formation assay of RKO and HT-29 cells with control, furanodienone or 5-FU. (c) Furanodienone induced G0/G1 phase arrest. RKO and HT-29 cells were exposed to furanodienone (0, 50, 100 and 150 μM) for 24 h followed by flow cytometric assay. The percentage of cell cycle distribution was showed as mean±S.D. from three independent experiments. The mRNA expression of p21Cip1, CDK 4, CDK 6 and cyclin D1 in RKO cells treated with furanodienone for 24 h were detected using RT-qPCR (d). The expression of G0/G1 phase-related proteins were analyzed by western blotting (e). *P<0.05, **P<0.01, ***P<0.0001, significantly different compared with control