Figure 6.
Furanodienone inhibits tumorigenesis of human colorectal xenograft in vivo. RKO cells were inoculated subcutaneously into the right flank of BALB/c-nu. When the average size of tumors reached 50 mm3, mice were randomly divided into two groups receiving intraperitoneal injections with 10% DMSO or furanodienone (2 mg/kg) every other day during 2 weeks. (a and b) Tumor sizes and body weights were measured on a weekly basis. (c) The level of cleaved caspase-3, p-JNK, p-P38 and p-ERK in tumor xenograft tissues was detected by western blot. (d) The apoptotic status in tumor tissues was assessed by TUNEL assay. HE staining was used to evaluated the histology. The expression of cleaved caspase-3, p-JNK, p-P38 and p-ERK was examined by immunohistochemistry, and mean optical density of which were quantified by Image Pro-Plus (IPP, Media Cybernetics, BD Biosciences, Mountain View, CA, USA). *P<0.05, significantly different compared with control