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. 2017 May 25;8(5):e2823. doi: 10.1038/cddis.2017.224

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The effects of Aroclor1254 on the barrier function of SC epithelium after inhibiting the caveolin-dependent endocytosis. The SCs were treated with or without Aroclor1254 in the presence or absence of CO as the regimen depicted in Supplementary Figure S2a. (a and b) The barrier function of SC epithelium measured by recording TER (a) or calculating the permeability of Na-F (b) across the cell monolayer. The Na-F permeability in the vehicle control on day 3 was arbitrarily set at 100%. CO treatment was found to partially resist the disruption of BTB by Aroclor1254. Each bar refers to mean±S.D. of n=3 experiments using different batches of SCs. **P<0.01, compared with the vehicle control group. ^◊P<0.05; ^◊◊P<0.01, compared with the CO group. ^#P<0.05; ^##P<0.01, compared with the Aroclor1254 group. (c) Immunoblot analysis of endocytosed JAM-A, N-cadherin, and β-catenin in SCs at different time points (0, 10, 30, 60 min) after cell surface biotinylation for 30 min in the presence of Aroclor1254 (or the mixture of Aroclor1254 and CO). Total biotinylated proteins at 0 min without stripping served as the positive control. Cell lysates without pull-down were analyzed by immunoblot to confirm identical levels of the tested protein between groups with GAPDH as the loading control. (d) Line and scatter graphs summarizing the result shown in c by calculating the percentage of endocytosed protein at each data point versus the total biotinylated protein. The endocytosis of JAM-A, N-cadherin, and β-catenin showed no difference between the CO-treated cells and CO+Aroclor1254-treated cells. *P<0.05; **P<0.01, compared with the vehicle control group. ^##P<0.01, compared with the Aroclor1254 group. (e) Immunofluorescence assay to observe the distribution of N-cadherin and β-catenin at SC–SC junctions on day 6 after Aroclor1254 treatment for 24 h in the presence or absence of CO, using Alexa Fluor 488-conjugated secondary antibody (green) with cell nuclei stained with DAPI (blue). Scale bar=25 μm, which applied to all micrographs in (e)