Figure 6.

PEDF is a direct target of miR-320c. (a) Schematic miR-320c putative target sites in the CDS of PEDF. (b) Percentage of specimens showing low or high miR-320c expression with the expression levels of PEDF. (c and d) Cells were transfected with miR-320c inhibitors for 48 h, and then performed WB or qRT-PCR analysis. (e) The sequence of PEDF-mut. (f) Indicated plasmids were co-transfected with NC or miR-320c mimics, respectively, for 48 h and then performed luciferase reporter assay, pmir-GLO was transfected as the internal control. (g) The schematic overview of the potential mechanism involved in PEDF-mediated EMT in NPC. Bars correspond to mean±standard deviation (S.D.), *P<0.05