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. 2017 Jun 29;8(6):e2906. doi: 10.1038/cddis.2017.142

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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miR-29a decreased 5-hmC levels by directly targeting TET methylcytosine dioxygenases. (a) miR-29a target sites predicted by TargetScan for human, mouse, and zebrafish TET-family members. (b) miR-29a significantly suppressed the luciferase activity of TET family containing a wild-type 3'-UTR, but showed no effect on the activity of TET family with a mutant 3'-UTR. (c and d) Following transfection with miR-29a vector, qRT-PCR and western blotting experiments showed the mRNA and protein levels of TET1, 2, and 3 in SMMC-7721 and HepG2 cells were significantly decreased compared with controls. (e) Global 5-hmC levels in HepG2-miR-29a cells and control miRNA were measured. (f) shTET1, shTET2, and shTET3 in HCCLM3-anti-miR-29a cells were determined (left). Global 5-hmC levels in shTET1, 2, and 3 and negative control groups were measured (right). Data are shown as mean±S.D. (n=3)