Figure 6.

Effects of MLN4924 on apoptosis and cell cycle progression in human NPC cell lines. (a,b) CNE2, S18, S26, 5-8Fand SUNE1 were treated with MLN4924 at 1 μM and 3 μM for 48 h, followed by FACS for apoptosis analysis. (c,d) The given cell lines were exposed to the indicated concentrations of MLN4924 48 h and then harvested for cell cycle profiling. (e–g) CNE2 and SUNE1 were lysed after MLN4924 treatment for 48 h, and western blot analysis to evaluate the expression of protein and β-actin as a loading control. Data are presented as means ±S.E.M. of three independent experiments. *P<0.05, **P<0.01 as compared with the controls